Author(s): CHEN Jia-wen, XU Lin, GAO Yao-zheng, SU Wei-heng, CHEN Wei-jin, College of Life Science, Jilin University, National Engineering Laboratory for, AIDS Vaccine, College of Life Science, Jilin University, Changchun Institute of Products Co., Ltd.
Pages: 1963-1966
Year: 2014 Issue:  16
Journal: Chinese Journal of New Drugs
Keyword:  EV71-EGFP virus infectious clone; RDS cell; arenobufagin; active ingredients;
Abstract: Objective: To study the inhibitory effect of the two main active ingredients of arenobufagin on EV71-EGFP virus infections clone to choose natural products that can inhibit EV71-EGFP infection and provide candidate drugs for prevention and treatment of hand-foot-mouth disease caused by EV71. Methods: RDS cells and the two main active ingredients from arenobufagin,Cinobufagin and Resibufogenin,in different dilution multiples were plated in 96-well plates at the same time,and EV71-EGFP was used to infect the cells. After 15 h,the plate were observed under inverted microscope for morphology. The fluorescence was tested by fluorescence microscope,and then the EGFP values were measured by a multimode microplate reader. Results: Compared with the control cells,the CPE caused by EV71-EGFP virus infection was reduced in the cells added with the two active ingredients of arenobufagin,but there was still morphology change in part of the cells. The density and vitality of RDS cells were increased,and the number of EV71-EGFP virus infectious clone in RDS cell was reduced effectively by the two ingredients. Conclusion: The two main active ingredients of arenobufagin have inhibitory effect on EV71-EGFP virus infection.