Study on the imbalance of peripheral blood Treg / Th17 cells in childhood aplastic anemia
Author(s): WANG Ningling, WANG Min, WANG Huiping, CHU Jinhua, WANG Yan, HUANG Lingling, YANG Linhai, XIE Zhiwei, TU Songji, Department of Paediatrics, the Second Affiliated Hospital of Anhui Medical University
Pages: 202-206
Year: 2014 Issue: 4
Journal: China Child Blood
Keyword: regulatory T cell; Th17 cell; Anemia; aplastic; IL-17; IL-6;
Abstract: Objective To investigate the role and clinical significance of Treg / Th17 cell imbalance in pathogenesis of childhood aplastic anemia. Methods The flow cytometry was used to evaluate the proportion of peripheral blood Treg cells and Th17 cells in childhood aplastic anemia and healthy children. The levels of plasma IL-17 and IL-6 were assayed by enzyme-linked immunosorbent assay(ELISA). Results The percentage of peripheral blood Th17 cells was increased significantly in aplastic anemia children compared with healthy controls [(1. 45 ± 0. 28)% vs(0. 45 ± 0. 10)% ]( P <0. 01),while the percentage of Treg cells and the ratio of Treg /Th17 was markedly lower [(4. 05 ±1. 07)% vs(6. 96 ± 0. 79)%;(2. 89 ± 0. 88) vs(15. 77 ± 2. 77)]( P < 0. 01). The levels of plasma IL-17 and IL-6 in childhood aplastic anemia were(185. 96 ± 40. 42)pg /mL and(20. 78 ± 5. 49)pg /mL,respectively,which were significantly increased compared with controls[(120. 47 ± 18. 39) pg /mL and(10. 44 ± 2. 51) pg/mL](P < 0.01). Furthermore,both plasma levels of IL-17 and IL-6 were positively correlated with the proportion of Th17( r = 0. 67,P < 0. 01; r = 0. 57,P < 0. 01),but the percentage of Treg cells had negative correlation with the plasma concentration of IL-6( r =- 0. 39,P <0. 05). Conclusions The imbalance between Treg cells and Th17 cells resulted from decreased ratio of Treg cells and increased ratio of Th17 cells,which may play a critical role in pathogenesis of childhood aplastic anemia,and the increase expression of IL-6 may be one of the factors causing the imbalance of Treg / Th17 cells.
Pages: 202-206
Year: 2014 Issue: 4
Journal: China Child Blood
Keyword: regulatory T cell; Th17 cell; Anemia; aplastic; IL-17; IL-6;
Abstract: Objective To investigate the role and clinical significance of Treg / Th17 cell imbalance in pathogenesis of childhood aplastic anemia. Methods The flow cytometry was used to evaluate the proportion of peripheral blood Treg cells and Th17 cells in childhood aplastic anemia and healthy children. The levels of plasma IL-17 and IL-6 were assayed by enzyme-linked immunosorbent assay(ELISA). Results The percentage of peripheral blood Th17 cells was increased significantly in aplastic anemia children compared with healthy controls [(1. 45 ± 0. 28)% vs(0. 45 ± 0. 10)% ]( P <0. 01),while the percentage of Treg cells and the ratio of Treg /Th17 was markedly lower [(4. 05 ±1. 07)% vs(6. 96 ± 0. 79)%;(2. 89 ± 0. 88) vs(15. 77 ± 2. 77)]( P < 0. 01). The levels of plasma IL-17 and IL-6 in childhood aplastic anemia were(185. 96 ± 40. 42)pg /mL and(20. 78 ± 5. 49)pg /mL,respectively,which were significantly increased compared with controls[(120. 47 ± 18. 39) pg /mL and(10. 44 ± 2. 51) pg/mL](P < 0.01). Furthermore,both plasma levels of IL-17 and IL-6 were positively correlated with the proportion of Th17( r = 0. 67,P < 0. 01; r = 0. 57,P < 0. 01),but the percentage of Treg cells had negative correlation with the plasma concentration of IL-6( r =- 0. 39,P <0. 05). Conclusions The imbalance between Treg cells and Th17 cells resulted from decreased ratio of Treg cells and increased ratio of Th17 cells,which may play a critical role in pathogenesis of childhood aplastic anemia,and the increase expression of IL-6 may be one of the factors causing the imbalance of Treg / Th17 cells.
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