The preparation and application of inactivated vaccine and its antiserum from eight common pathogens of Flatfish
Author(s): Gan Lingling, Wang Weifang, Gao Chunren, Lei Jilin
Pages: 21-25
Year: 2014 Issue: 9
Journal: Engineering Science
Keyword: Flatfish; vaccine; antiserum; pathogen;
Abstract: In this study,formal dehyde was used to inactivate Edwardsiella tarda,Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri,Aeromonas hydrophila,in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antise-rum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune,and the level of antibody is research highest in the tenth week which is inoculated inactivated vac-cine of Edwardsiella tarda,and the highest titer of other vaccines are 1:102 400,1:6 400, 1:6 400,1:12 800,1:12 800,1:6400,1:6 400,1:12 800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum,and it is very weak when the bacteria react with other antiserum.
Pages: 21-25
Year: 2014 Issue: 9
Journal: Engineering Science
Keyword: Flatfish; vaccine; antiserum; pathogen;
Abstract: In this study,formal dehyde was used to inactivate Edwardsiella tarda,Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri,Aeromonas hydrophila,in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antise-rum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune,and the level of antibody is research highest in the tenth week which is inoculated inactivated vac-cine of Edwardsiella tarda,and the highest titer of other vaccines are 1:102 400,1:6 400, 1:6 400,1:12 800,1:12 800,1:6400,1:6 400,1:12 800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum,and it is very weak when the bacteria react with other antiserum.
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