Cytological and AFLP Analysis of the Wheat-Rye Somaclonal Variation with Higher Chromosome Pairing Rate
Author(s): SU Yarui, GAO Xiaofeng, CAO Jinguo, LI Li, LI Suoping, Henan University College of Life Science, Henan University Pharmaceutical College
Pages: 575-581
Year: 2014 Issue: 5
Journal: Journal of Henan University(Natural Science)
Keyword: wheat-rye; somaclonal variation; GISH; AFLP;
Abstract: Previous cytological analysis of the wheat × rye hybrids revealed a high frequency of bivalents at metaphase I in some somaclonal variation in the embryogenic cell culture.In order to determine the molecular nature of the change,the hybrids non-regenerated from tissue culture and the somaclonal variation with higher number of chromosome pairing were examined by genomic in situ hybridization(GISH)and the amplified fragment length polymorphism(AFLP)technique.It is indicated that somaclonal variation gave rise to pairing configuration of W-R and R-R.Furthermore,the source and type of genomic changes were investigated that extensive genomic changes were observed as early as F1 in all hybrids,and that the major variation was the parentssequence elimination.Moreover,the change of somaclonal variation with higher number of chromosome pairing was much larger than that of the hybrids non-regenerated from tissue culture.The result suggested that the somaclonal variation could increase the genomic changes.It is the first time to report the cytology and the molecular biology foundation of high frequent pairing variation.
Pages: 575-581
Year: 2014 Issue: 5
Journal: Journal of Henan University(Natural Science)
Keyword: wheat-rye; somaclonal variation; GISH; AFLP;
Abstract: Previous cytological analysis of the wheat × rye hybrids revealed a high frequency of bivalents at metaphase I in some somaclonal variation in the embryogenic cell culture.In order to determine the molecular nature of the change,the hybrids non-regenerated from tissue culture and the somaclonal variation with higher number of chromosome pairing were examined by genomic in situ hybridization(GISH)and the amplified fragment length polymorphism(AFLP)technique.It is indicated that somaclonal variation gave rise to pairing configuration of W-R and R-R.Furthermore,the source and type of genomic changes were investigated that extensive genomic changes were observed as early as F1 in all hybrids,and that the major variation was the parentssequence elimination.Moreover,the change of somaclonal variation with higher number of chromosome pairing was much larger than that of the hybrids non-regenerated from tissue culture.The result suggested that the somaclonal variation could increase the genomic changes.It is the first time to report the cytology and the molecular biology foundation of high frequent pairing variation.
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