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Issue:
Allele-specific PCR and Its Application in Forensic Science
Author(s):
NIE Yan-chai
,
WANG Bin
,
ZHAO Zi-qin
,
ZHOU Huai-gu
Pages:
282
-
287
Year:
2014
Issue:
4
Journal:
Journal of Forensic Medicine
Keyword:
forensic genetics
;
polymerase chain reaction
;
review [publication type]
;
allele specific
;
Abstract:
等位基因特异性PCR(allele-specific polymerase chain reaction,AS-PCR)是一种基于等位基因特异性引物引导的PCR技术,可以有效地分析单核苷酸多态性(SNP),包括碱基的转换、颠换以及插入/缺失多态性,在疾病研究、分子诊断以及法医物证学研究中具有很好的应用价值.本文系统地综述了AS-PCR技术的原理、检测手段、改进方法及在常染色体、Y染色体和线粒体SNP等领域的研究成果,探讨其法医学应用价值.
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