Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation
Author(s): GUO Ying, LIU Bao-sheng, LI Zhi-yun, ZHANG Li-hui, LYU Yun-kai
Pages: 1114-1119
Year: 2014 Issue: 9
Journal: Chinese Journal of Luminescence
Keyword: bovine serum albumin; binding constant; combination of points; comparative study;
Abstract: In Tris-HCl buffer solution (pH = 7. 4), the interactions of moxifloxacin, norfloxacin, ofloxacin, enrofloxacin, lomefloxacin, and ciprofloxacin with bovine serum albumin were studied, respectively. Using modified Scatchard equation, Lineweaver-Burk double reciprocal equation, double logarithmic equation, modified Stern-Volmer equation, and improved double logarithmic equation, the binding constants and binding points of drugs with bovine serum albumin were calcu-lated, respectively. For the binding reaction of drug-protein ratio of 1∶ 1, the calculation results are very close except modified Scatchard equation, and the improved double logarithmic equation is the most accurate.
Pages: 1114-1119
Year: 2014 Issue: 9
Journal: Chinese Journal of Luminescence
Keyword: bovine serum albumin; binding constant; combination of points; comparative study;
Abstract: In Tris-HCl buffer solution (pH = 7. 4), the interactions of moxifloxacin, norfloxacin, ofloxacin, enrofloxacin, lomefloxacin, and ciprofloxacin with bovine serum albumin were studied, respectively. Using modified Scatchard equation, Lineweaver-Burk double reciprocal equation, double logarithmic equation, modified Stern-Volmer equation, and improved double logarithmic equation, the binding constants and binding points of drugs with bovine serum albumin were calcu-lated, respectively. For the binding reaction of drug-protein ratio of 1∶ 1, the calculation results are very close except modified Scatchard equation, and the improved double logarithmic equation is the most accurate.
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