CLONING AND EXPRESSION OF GENE VASA DURING OVARIAN DEVELOPMENT CYCLE IN EXOPALAEMON CARINICAUDA
Author(s): XU Wen-Fei, LIU Ping, LI Ji-Tao, LI Jian, CHEN Ping, Ocean University of Dalian, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
Pages: 574-582
Year: 2014 Issue: 3
Journal: Oceanologia et Limnologia Sinica
Keyword: Exopalaemon carinicauda; vasa; gene cloning; mRNA expression; ovarian development cycle;
Abstract: The vasa gene encodes DEAD box family protein of ATP-dependent RNA helicase,and it plays a crucial role in primordial germ cell proliferation.In this report,a vasa gene was isolated from Exopalaemon and named Ec-vasal carinicauda using reverse transcription polymerase chain reaction(RT-PCR) coupled with rapid amplification of cDNA ends(RACE) approaches.The Ec-vasa cDNA sequence comprises 2516bp with an open reading frame of 1 800bp encoding 600 amino acids.The deduced amino acid sequence contains 8 conserved motifs of DEAD-box family protein.Comparisons in the deduced amino acid sequence with those of other invertebrates revealed the highest homology(80%) with Macrobrachium nipponense.In the organs of the adult,the Ec-vasa transcripts were detected in ovary.In the ovarian development cycle,the highest mRNA expression level of vasa gene was found instageⅠ,and then the transcripts decreased with ovarian development.As the time progressed,the expression level of Ec-vasa reached the lowest level at stage Ⅳ.These results indicate that Ec-vasa gene may play an important role in oogenesis during ovarian development cycle,and it was expected as an effective molecular marker to the origin of PGCs and gonadogenesis.
Pages: 574-582
Year: 2014 Issue: 3
Journal: Oceanologia et Limnologia Sinica
Keyword: Exopalaemon carinicauda; vasa; gene cloning; mRNA expression; ovarian development cycle;
Abstract: The vasa gene encodes DEAD box family protein of ATP-dependent RNA helicase,and it plays a crucial role in primordial germ cell proliferation.In this report,a vasa gene was isolated from Exopalaemon and named Ec-vasal carinicauda using reverse transcription polymerase chain reaction(RT-PCR) coupled with rapid amplification of cDNA ends(RACE) approaches.The Ec-vasa cDNA sequence comprises 2516bp with an open reading frame of 1 800bp encoding 600 amino acids.The deduced amino acid sequence contains 8 conserved motifs of DEAD-box family protein.Comparisons in the deduced amino acid sequence with those of other invertebrates revealed the highest homology(80%) with Macrobrachium nipponense.In the organs of the adult,the Ec-vasa transcripts were detected in ovary.In the ovarian development cycle,the highest mRNA expression level of vasa gene was found instageⅠ,and then the transcripts decreased with ovarian development.As the time progressed,the expression level of Ec-vasa reached the lowest level at stage Ⅳ.These results indicate that Ec-vasa gene may play an important role in oogenesis during ovarian development cycle,and it was expected as an effective molecular marker to the origin of PGCs and gonadogenesis.
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