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ACID DIGESTION IN TURBOT SCOPHTHALMUS MAXIMUS BASED ON H~+/K~+ ATPase
Author(s): 
Pages: 644-650
Year: Issue:  3
Journal: Oceanologia et Limnologia Sinica

Keyword:  Scophthalmus maximusH+/K+ ATPaseexpression analysisdigestion;
Abstract: Gastric proton pump(H+/K+ ATPase) is a key enzyme involved in gastric acid secretion.We isolated a full-length cDNA sequence of α subunit of H+/K+ ATPase from gastric tissue of turbot Scophthalmus maximus through RT-PCR and RACE(Rapid amplification of cDNA ends).Results indicate that H+/K+ ATPase cDNA was 3467 bp from which 988 amino acids were deduced.The nucleotied sequence of α subunit of H+/K+ ATPase shared high homology with Siniperca chautsi(89%).Phylogenetic analysis showed that H+/K+ ATPase was species-specific in evolution.The initial transcripts H+/K+ ATPase were detected in 22 days post hatching(dph),after gastric glands(16 dph) were formed.This suggested that the morphologic development of gastric glands was not synchronous with their functional development.In addition,H+/K+ ATPase mRNAs were expressed highly in esophagus.We surmised that the turbot stomach was reduced to a dilatation of esophagus,and that esophagus retained its ability to express pepsinogen.The distribution of tPGA and tPGC transcripts in the turbot was investigated using in-situ hybridization.The results show that tPGA and tPGC mRNA were first detected in the esophagus and cardiac region of the stomach.This study provided a basis for understanding the digestive mechanisms in teleost.
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