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INFLUENCE OF SEVERAL COMMON PRESERVATION METHODS ON THE BIOMASS OF ZOOPLANKTON
Author(s): 
Pages: 480-486
Year: Issue:  3
Journal: Oceanologia et Limnologia Sinica

Keyword:  Jiaozhou BayZooplanktonPreservationbiomass;
Abstract: 5% formalin,-20°C freeze,and liquid nitrogen are common methods used to preserve zooplankton.Taking Calanus sinicus and Sagitta crassa as target organisms,and wet weight,dry weight,carbon,and nitrogen content as biomass parameters,influences of the above preservation conditions on zooplankton biomass are studied.For Calanus sinicus preserved in formalin for 2 months,the wet weight,dry weight,carbon,and nitrogen content reduced to 81.03%,14.02%,7.02% and 1.13% of the fresh weight,respectively; when frozen at –20°C,they reduced to 66.56%,22.34%,10.30%,and 2.14%,and when preserved in liquid nitrogen,reduced to 91.85%,26.65%,11.25% and 2.42% of the fresh weight,respectively.For Sagitta crassa,the parameters are 104%,9%,3.49% and 0.69% of the fresh weight when preserved in formalin for two month,and 73.15%,12.39%,4.82% and 1.02% when frozen at-20°C,and 70.72%,11.22%,5.23% and 1.13% when preserved in liquid nitrogen,respectively.It was found that the zooplankton biomass decreased quickly at begining,and then tend to stabilize.Preservation in formalin showed the greatest influence on zooplankton biomass,following by in liquid nitrogen and in-20°C freeze.These above three methods of preservation caused smaller impacts on Calanus sinicus than on Sagitta crassa.
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