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EFFECT OF AMMONIA STRESS ON IMMUNITY INDICATORS OF JUVENILE MUGIL CEPHALUS
Author(s): 
Pages: 529-535
Year: Issue:  3
Journal: Oceanologia et Limnologia Sinica

Keyword:  sammonia exposurejuvenile Mugil cephalusmalondialdehyde(MDA)Na+-K+-ATPratio of RNA/DNAmRNA expression;
Abstract: To evaluate the effects of ammonia on immunity and metabolism of juvenile Mugil cephalus,their tissues were exposed to different ammonia levels: 0.35(natural seawater or control),0.7,1.5,and 3.0 mg/L for 20days and sampled randomly on Days 0,5,10,15,and 20.The results indicate that as the exposure time lasted,malondialdehyde(MDA) content in liver and gill decreased after an initial increase.Ammonia concentration was positively correlated to the MDA content significantly.The relationship of ammonia stress and MDA content in liver and gill is somewhat tissue and sequence specific.The activity of Na+-K+-ATP in liver and gill decreased after an initial increase with exposure time lasted; and ammonia concentration showed significant negative correlation to Na+-K+-ATP activity.The ratio of RNA/DNA in muscle slightly increased but lower than the control except at 3.0 mg/L(P>0.05) treatment group.The mRNA expression of Na+-K+-ATPase increased till Day 5,then resumed to the level same as control after Day 20 except for 3.0 mg/L group staying at higher level than the control’s.The variation trends of mRNA expression of Na+-K+-ATPase were largely the same to the activity of Na+-K+-ATP.The results indicate that ammonia-nitrogen stress in a certain concentration for a long time could disturb the antioxidant immune system and nucleic acid metabolism in muscle of juvenile Mugil cephalus.
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