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EFFECT OF VIBRIO ANGUILLARUM ON MORPHOLOGY AND IMMUNOLOGICAL FUNCTION OF BLOOD CELLS IN SCAPHARCA BROUGHTONII
Author(s): 
Pages: 536-541
Year: Issue:  3
Journal: Oceanologia et Limnologia Sinica

Keyword:  Scapharca broughtoniiVibrio anguillarumblood cellsanticoagulant;
Abstract: We studied morphological change of blood cells in hemolymph of Scapharca broughtonii after immunostimulation with pathogenic Vibrio anguillarum observed under oil immersion lens of Leica microscope in two regular anticoagulants.Morphological change of red blood cells,white blood cells,and thrombus cells were common in hemolymph due possibly to stress response of immune defense.Red cells often showed amitosis and split,and were often involved in phagocytosis.Deformation,phagocytosis,and fusion were common for white cells,and white cells were involved in the clots formation for their viscous mobility surface.Thrombocytes had adhesion and coagulation properties,and occasionally,they were found bacteria adhesion and phagocytosis.In addition,we tested the anticoagulation effect by the two anticoagulants,showing that the two anticoagulants could prevent white cells or thrombocytes from coagulation by changing their cell-surface characteristics.Therefore,they could be used as reliable anticoagulants.The immunological function played by each type of blood cells is very clear in cellular immunity.The morphological changes and immunological function enriched our understanding on blood immunological mechanism of Scapharca broughtonii,and may help develop anticoagulants.
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