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Effect of hOGG1 expression level on oxidative DNA damage among workers exposed to nickel in stainless steel production environment
Author(s): 
Pages: 578-581
Year: Issue:  8
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Stainless steel productionhOGG1 expression levelDNA Oxidative damage;
Abstract: Objective To study the excision repair capacity of human 8-oxoguanine DNA N-glycosylase 1 (hOGG1) for 8-OH-dG and the oxidative DNA damage among workers exposed to nickel in stainless steel production environment.Methods A total of 231 workers exposed to nickel in a stainless steel production enterprise were recruited as nickel exposure group,and another 75 water pump workers in that enterprise were recruited as control group.The workplace occupational hazard factors were determined.Double-antigen sandwich ELISA was used to determine urinary 8-OH-dG level; RT-PCR was used to determine hOGG1 mRNA level.Pearson correlation was used to analyze the correlation between urinary 8-OH-dG level and hOGG1 mRNA level.Results Level of 8-OH-dG was compared between different types of nickel-exposed workers and control workers; rolling mill workers showed no significant difference from the control group (P>0.05),while steelmaking workers and steel slag disposing workers showed significant differences from the control group (P< 0.05).Level of 8-OH-dG was also compared between nickel-exposed workers with different working years and control workers; nickel-exposed workers with 0~5 and 6~10 working years showed no significant differences from the control group (P>0.05),while other exposed workers showed significant differences from the control group (P<0.05).Different types of nickel-exposed workers all showed significant differences from the control group in hOGG 1 mRNA level (P<0.05).Nickel-exposed workers with 0~5 working years showed no significant difference from the control group in hOGG 1 mRNA level (P>0.05),while other exposed workers showed significant differences from the control group (P<0.05).Pearson correlation analysis showed that urinary 8-OH-dG level was positively correlated with hOGG1 mRNA level (r=0.993) in different types of nickel-exposed workers,and the correlation was significant at α=0.01 (P<0.05); urinary 8-OH-dG level also showed a positive correlation with hOGG1 mRNA level in nickel-exposed workers with different working years (r=0.968),and the correlation was significant at α=0.01 (P<0.05).Conclusion Exposure to nickel increases oxidative DNA damage among steel workers,and hOGG1 shows active excision repair capacity for 8-OH-dG.
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