Author(s): DAI Xiaojun, ZHANG Xiaochun, SUN Weidong, JI Xiaodong, LIU Moxiang, LIU Yanqing, Yangzhou Hosp Affil, Nanjing Univ of Trad Chineses Med, Yangzhou Hosp of Trad Chineses Med, Coll of Med, Yangzhou Univ
Pages: 11-14
Year: 2014 Issue:  2
Journal: Journal of Yangzhou University(Agricultural and Life Science Edition)
Keyword:  stomach neoplasms; ginsenoside Rg3; neoplasms transplantation; nude;
Abstract: NUGC-4cell lines with stable expression of red fluorescent protein(red fluorescent protein,RFP)were established by transfection via lentiviral vector.The gastric cancer models were developed by surgical orthotopic implantation of red fluorescent protein(RFP)-expressing NUGC-4tumor in nude mice.The dynamic growth of xenografted tumor was observed using fluorescence imaging system in vivo.After treated with VEGFR3-mediated immune-nanoemulsion of ginsenoside Rg3(VRIN),the volume and weight of transplanted tumors in nude mice were measured.Morphology of transplanted tumor tissues was observed by HE staining.Results showed that stable RFP-expressing gastric cancer cell lines and xenografted tumor model were obtained.Optical imaging showed that the fluorescent intensity of VRIN treated group was apparently lower than that of the control group which was treated with saline.As compared to the control group,the volume and weight of tumors in VRIN treated group were significantly decreased(P<0.01).VRIN treated group induced obvious necrosis of tumor cells in implanted tumor tissues.VRIN can inhibit the growth of human gastric cancer cell transplantation tumor in nude mice.