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Study of Banana Cauloid Explant Callus Induction
Author(s): 
Pages: 6167-6169
Year: Issue:  19
Journal: Journal of Anhui Agricultural Sciences

Keyword:  BananaEmbryogenic callusSuspension culture;
Abstract: [目的]研究香蕉假茎外植体愈伤组织的最佳培养基.[方法]以香蕉组培无菌苗假茎为外植体,接种在含不同浓度配比激素的固体培养基中进行诱导培养,再将培养获得的胚性愈伤组织接种到液体培养基中进行悬浮培养.[结果]使用低浓度噻苯隆(TDZ)诱导出胚性愈伤组织的效果最好,最佳培养基为T2:MS+0.02 mg/L TDZ +7 mg/L2,4-D.[结论]悬浮培养使用ZT2能较早建立ECS,且继代效果较好.
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