Author(s): WANG Ai-min, YAN Yan, LAN Bo, LIAO Shang-gao, WANG Yong-lin, LI Yong-jun, Engineering Research Center for the Development and Application of Ethnic Medicine and, TCM, School of Pharmacy, Guiyang Medical College, Department of Pharmacy, Guizhou Provincial People’s Hospital, Guizhou Provincial Key Laboratory of Pharmaceutics, Guiyang Medical College
Pages: 2051-2055
Year: 2014 Issue:  11
Journal: China Journal of Chinese Materia Medica
Keyword:  Bletillae Rhizoma; multiple chemical markers; ultra performance liquid chromatography;
Abstract: A UPLC method has been developed in the current investigation for simultaneous determination of nine chemical markers of Bletilla striata,4-hydroxymethylphenyl β-D-glucoside,blestroside,dactylorhin A,militarine,dihydrophenanthrene 5,gymnoside V,dihydrophenanthrene 1,benzylphenanthrene 3 and gymnosides Ⅸ.Separation was performed at 45 ℃ on an ACQUITY UPLC BEH C18column(2.1 mm × 150 mm,1.7 μm) with a gradient solvent system of acetonitrile-water as the mobile phase.The flow rate was 0.3 mL·min-1,the detection wavelength was 280 nm.The results showed that the nine chemical markers could be well resolved and that in the selected linear range,all calibration curves of the nine chemical markers showed good linearity(r≥0.999 3).The recoveries(n = 6) were in the range of 98.15% ~ 102.2% and RSDs were between 2.1% ~ 3.6%.The data suggested that the developed UPLC-UV method had good reproducibility,robustness,and accuracy,which was suitable for the quality control of Bletilla striata.Applications of the method showed that the nine chemical markers had higher contents in the wild B.striata than in the cultivated ones.