Establishment and effectiveness comparison of mouse models of acute lung injury by intratracheal instillation and aerosol inhalation
Author(s): YUAN Shaoxin, KONG Yaxian, LI Rui, LIU Sanhai, ZHU Anni, QIAO Yanxue, ZHANG Jianping
Pages: 163-167
Year: 2014 Issue: 2
Journal: Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version)
Keyword: Lipopolysaccharide; Intratracheal instillation; Aerosol inhalation; Acute lung injury;
Abstract: Objective To compare the mouse models of acute lung injury (ALI) established by intratracheal instillation (IT) and aerosol inhalation (IH) of lipopolysaccharide (LPS), and to investigate which method was more effective. Methods Twenty male mice were randomly divided into normal saline (NS)-IT group, NS-IH group, LPS-IT group and LPS-IH group. The mice of LPS-IT and LPS-IH groups were exposed to LPS via intratracheal instillation and aerosol inhalation, respectively. The analysis of lung wet/dry weight (W/D) ratio, total protein concentration of bronchoalveolar lavage fluid (BALF), inflammatory cytokines in BALF and multiple cell countings in BALF were performed 5 h after exposed. Results The lung W/D ratio, total protein concentration of BALF, inlfammatory cytokines such as TNF-α, IL-6, MCP-1 and IL-1βin BALF, and the amount of neutrophils in BALF were signiifcantly increased in the LPS-IT and LPS-IH groups compared with the NS-IT and NS-IH groups (P all < 0.05). Compared with the intratracheal instillation method, the aerosol inhalation method appeared to have lower background inlfammation in the controls and less inter-animal variability. Conclusion The aerosol inhalation is proved to be more effective to establish the mouse model of ALI.
Pages: 163-167
Year: 2014 Issue: 2
Journal: Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version)
Keyword: Lipopolysaccharide; Intratracheal instillation; Aerosol inhalation; Acute lung injury;
Abstract: Objective To compare the mouse models of acute lung injury (ALI) established by intratracheal instillation (IT) and aerosol inhalation (IH) of lipopolysaccharide (LPS), and to investigate which method was more effective. Methods Twenty male mice were randomly divided into normal saline (NS)-IT group, NS-IH group, LPS-IT group and LPS-IH group. The mice of LPS-IT and LPS-IH groups were exposed to LPS via intratracheal instillation and aerosol inhalation, respectively. The analysis of lung wet/dry weight (W/D) ratio, total protein concentration of bronchoalveolar lavage fluid (BALF), inflammatory cytokines in BALF and multiple cell countings in BALF were performed 5 h after exposed. Results The lung W/D ratio, total protein concentration of BALF, inlfammatory cytokines such as TNF-α, IL-6, MCP-1 and IL-1βin BALF, and the amount of neutrophils in BALF were signiifcantly increased in the LPS-IT and LPS-IH groups compared with the NS-IT and NS-IH groups (P all < 0.05). Compared with the intratracheal instillation method, the aerosol inhalation method appeared to have lower background inlfammation in the controls and less inter-animal variability. Conclusion The aerosol inhalation is proved to be more effective to establish the mouse model of ALI.
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