Isolation and identification of cellulolytic anaerobic fungi and their associated methanogens from Holstein Cow
Author(s): Meizhou Sun, Wei Jin, Yuanfei Li, Shengyong Mao, Yanfen Cheng, Weiyun Zhu, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University
Pages: 563-571
Year: 2014 Issue: 5
Journal: Acta Microbiologica Sinica
Keyword: anaerobic fungi; methanogens; co-cultures;
Abstract: [Objective] We studied the microbial interaction between anaerobic fungi and methanogens in the rumen of Holstein Cow. [Methods] Co-cultures of anaerobic fungi with indigenously associated methanogen were isolated by Hungate roll-tube technique. The anaerobic fungi were identified by morphology and 4 ’,6 diamidino-2-phylindole nucleus staining and the methanogens were identified by 16S rRNA gene sequencing. [Results]A total of 28 co-cultures of anaerobic fungus with indigenously associated methanogen were obtained. The anaerobic fungi in the co-cultures were identified as monocentric genera Piromyces,Neocallimastix and Caeomyces. The indigenously associated methanogens were Methanobrevibacter olleyae like and Methanobrevibacter thaueri like strains. Four different phylotypes of fungus-methanogen co-cultures were obtained, which were Piromyces/Methanobrevibacter olleyae like strains, Neocallimastix /Methanobrevibacter olleyae like strains, Neocallimastix/Methanobrevibacter thaueri like strains and Caecomyces /Methanobrevibacter olleyae like strains. [Conclusion]Our study isolated and identified 28 co-cultures of anaerobic fungus and associated methanogens,which provided new materials for further study the mechanism of methane emission in the rumen.
Pages: 563-571
Year: 2014 Issue: 5
Journal: Acta Microbiologica Sinica
Keyword: anaerobic fungi; methanogens; co-cultures;
Abstract: [Objective] We studied the microbial interaction between anaerobic fungi and methanogens in the rumen of Holstein Cow. [Methods] Co-cultures of anaerobic fungi with indigenously associated methanogen were isolated by Hungate roll-tube technique. The anaerobic fungi were identified by morphology and 4 ’,6 diamidino-2-phylindole nucleus staining and the methanogens were identified by 16S rRNA gene sequencing. [Results]A total of 28 co-cultures of anaerobic fungus with indigenously associated methanogen were obtained. The anaerobic fungi in the co-cultures were identified as monocentric genera Piromyces,Neocallimastix and Caeomyces. The indigenously associated methanogens were Methanobrevibacter olleyae like and Methanobrevibacter thaueri like strains. Four different phylotypes of fungus-methanogen co-cultures were obtained, which were Piromyces/Methanobrevibacter olleyae like strains, Neocallimastix /Methanobrevibacter olleyae like strains, Neocallimastix/Methanobrevibacter thaueri like strains and Caecomyces /Methanobrevibacter olleyae like strains. [Conclusion]Our study isolated and identified 28 co-cultures of anaerobic fungus and associated methanogens,which provided new materials for further study the mechanism of methane emission in the rumen.
Related Articles