Construction and expression of prokaryotic expression vector of M gene of porcine reproductive and respiratory syndrome virus
Author(s): LIU Cheng-qian, WANG Jing, YU Zong-xing, LI Hong, YI Jian-zhong, CHEN Lei
Pages: 22-25
Year: 2014 Issue: 2
Journal: Acta Agriculturae Shanghai
Keyword: PRRSV; M gene; Prokaryotic expression; Expression vector; Recombinant protein;
Abstract: 根据GenBank上已公布的猪繁殖与呼吸综合征病毒(PRRSV)核酸序列设计1对引物,用RT-PCR方法扩增出去除部分疏水区的PRRSV-M基因,并将其克隆到原核表达载体PET-30a上,得到重组质粒PET-30a-M.将重组质粒转化大肠杆菌transetta(DE3)感受态细胞,经IPTG诱导表达、SDS-PAGE电泳及Western blot分析,显示重组蛋白分子量约15.7 kD,且此蛋白能与His标签单克隆抗体发生特异性反应.
Pages: 22-25
Year: 2014 Issue: 2
Journal: Acta Agriculturae Shanghai
Keyword: PRRSV; M gene; Prokaryotic expression; Expression vector; Recombinant protein;
Abstract: 根据GenBank上已公布的猪繁殖与呼吸综合征病毒(PRRSV)核酸序列设计1对引物,用RT-PCR方法扩增出去除部分疏水区的PRRSV-M基因,并将其克隆到原核表达载体PET-30a上,得到重组质粒PET-30a-M.将重组质粒转化大肠杆菌transetta(DE3)感受态细胞,经IPTG诱导表达、SDS-PAGE电泳及Western blot分析,显示重组蛋白分子量约15.7 kD,且此蛋白能与His标签单克隆抗体发生特异性反应.
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