Author(s): WANG Qing-feng, MA Ning, WEN Peng, MA Tao, WANG Zhen, Department of Orthopedics, Ningxia People’s Hospital
Pages: 1442-1445
Year: 2014 Issue:  8
Journal: Biomagnetism
Keyword:  Human bone morphogenetic protein; Nucleus pulposus cells; Cell proliferation; Sulfated glycosaminoglycan; Collagen type Ⅱ;
Abstract: Objective: To investigate the primary cultured rabbit nucleus pulposus cells transfected with adenovirus vector mediated human bone morphogenetic protein-7 effect on its biological activity after(hBMP-7) gene. Methods: Depending on nucleus pulposus cells transfected way, into hBMP7 group, LacZ group, untransfected three groups, hBMP7 group using adenoviral vector-mediated transfection hBMP7, LacZ transfected with LacZ gene, not transfected genes without any transfection. Comparison of the three groups of cell proliferation, sulfate glycosaminoglycan((GAG) production, whether the difference in type Ⅱ collagen production. Results: After treatment and transfection time on cell proliferation, GAG production, type Ⅱ collagen production had interaction(P<0.05), hBMP7 cell proliferation, GAG production, type Ⅱ collagen production is higher than LacZ group, the non-transfected group, the difference was statistically significant(P<0.05), cell proliferation LacZ group and untransfected group, GAG production, Ⅱ collagen production was no significant difference(P>0.05). Conclusion: hBMP-7 gene transfection can increase the proliferation of nucleus pulposus cells and promote their produce extracellular matrix.