Efficacy and stability of maraviroc gel as a microbicide candidate in vit ro
Author(s): LI Liang-Feng, BEN Yin-Yin, LI Liang-Zhu, ZHANG Xiao-Yan
Pages: 102-106
Year: 2014 Issue: 2
Journal: Journal of Microbes and Infection
Keyword: Maraviroc; Hydroxyethyl cellulose; Human immunodeficiency virus; Microbicide;
Abstract: To study the efficacy and stability of maraviroc gel in vitro , the cytotoxicity of maraviroc in phosphate buffered saline (PBS) or in 0 .015% hydroxyethyl cellulose (HEC) were evaluated in TZM-bl and Jurkat-T cells .Meanwhile ,two subtypes of human immunodeficiency virus (HIV) pseudoviruses (SVPB16 and SVPC12) were used to evaluate the anti-HIV activity in vitro ,the half maximal inhibitory concentration (IC50 ) or 90% maximal inhibitory concentration (IC90 ) of maraviroc gel were assessed . Furthermore , maraviroc was dissolved in 1 .5% HEC with a final concentration of 6 mmol/L ,and then maraviroc gel was stored at 4 ℃ ,room temperature ,30 ℃ and 40 ℃ ,respectively ,in 75% relative humidity for 1 to 8 weeks . The anti-HIV activity was tested weekly .The data showed that maraviroc gel had nearly same cytotoxicity as maraviroc in PBS .HEC did not weaken the ant-HIV activity of maraviroc .Inversely ,HEC might slightly enhance the anti-HIV efficacy of maraviroc .Maraviroc gel was stable for at least 8 weeks even at 40 ℃ .It is concluded that maraviroc gel has good efficacy and stability as a microbicide candidate .
Pages: 102-106
Year: 2014 Issue: 2
Journal: Journal of Microbes and Infection
Keyword: Maraviroc; Hydroxyethyl cellulose; Human immunodeficiency virus; Microbicide;
Abstract: To study the efficacy and stability of maraviroc gel in vitro , the cytotoxicity of maraviroc in phosphate buffered saline (PBS) or in 0 .015% hydroxyethyl cellulose (HEC) were evaluated in TZM-bl and Jurkat-T cells .Meanwhile ,two subtypes of human immunodeficiency virus (HIV) pseudoviruses (SVPB16 and SVPC12) were used to evaluate the anti-HIV activity in vitro ,the half maximal inhibitory concentration (IC50 ) or 90% maximal inhibitory concentration (IC90 ) of maraviroc gel were assessed . Furthermore , maraviroc was dissolved in 1 .5% HEC with a final concentration of 6 mmol/L ,and then maraviroc gel was stored at 4 ℃ ,room temperature ,30 ℃ and 40 ℃ ,respectively ,in 75% relative humidity for 1 to 8 weeks . The anti-HIV activity was tested weekly .The data showed that maraviroc gel had nearly same cytotoxicity as maraviroc in PBS .HEC did not weaken the ant-HIV activity of maraviroc .Inversely ,HEC might slightly enhance the anti-HIV efficacy of maraviroc .Maraviroc gel was stable for at least 8 weeks even at 40 ℃ .It is concluded that maraviroc gel has good efficacy and stability as a microbicide candidate .
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