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Effect of pegylation recombinant human growth hormone on secretion function of pancreas islet in hypophysectomized rats and its mechanism
Author(s): 
Pages: 465-470
Year: Issue:  5
Journal: World Phytomedicines

Keyword:  polyethylene glycol recombinant human growth hormonerecombinant human growth hormonehypophysectomized ratinsulin resistance;
Abstract: Objective To observe whether the long-acting growth hormone polyethylene glycol recombinant human growth hormone(PEG-rhGH) causing insulin resistance or not, and the differences with the short-acting growth hormone recombinant human growth hormone(rhGH). Methods Sprague-Dawley young rats(106 rats) weighing 60 — 80 g were underwent hypophysectomy via parapharyngeal approach, and 18 rats with Sham operation were selected into the control group. Fifty-four qualified rats were randomly divided into the model, rhGH, and PEG-rhGH groups after two weeks, which were administered with saline(0.25 mg·kg-1·d-1), rhGH(0.25 mg·kg-1·d-1), and PEG-rhGH(1.4 mg·kg-1·week-1), respectively. After treatment for 4 weeks, glucose tolerance test and insulin release test were performed to calculate area under the curve insulin(AUCI), area under the curve glucose(AUCG), and homeostasis model assessment(HOMA-IR). Serum somatostatin(SS) levels were determined. Immunohistochemistry displayed the insulin(INS), glucagon(GLU), SS, and pancreatic polypeptide(PP). Results Glucose tolerance test and insulin release test indicated that HOMA-IR in PEG-rhGH group declined compared with those in the control and model groups(P < 0.05), and there were no difference between rhGH and PEG-rhGH groups. The value of AUCI/AUCG in PEG-rhGH group was higher than that of rhGH group without significant difference. Protein expression of GLU indicated no difference among PEG-rhGH, control, and model groups. The INS expression in PEG-rhGH group increased compared with the control group(P<0.05). SS and PP expression in PEG-rhGH group had no difference compared with that in rhGH group, and there was no significant difference of SS in serum among each group. Conclusion Insulin resistance and decreased islet β cell secretory capacity are not observed, and no significant effects of PEG-rhGH on secretion function of pancreas islet in hypophysectomized rats are observed.
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