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Determination of pulegone in Liujing Toutong Tablets by RP-HPLC
Author(s): 
Pages: 500-502
Year: Issue:  5
Journal: World Phytomedicines

Keyword:  Liujing Toutong TabletspulegoneHPLC;
Abstract: Objective To establish an HPLC method for the determination of pulegone in Liujing Toutong Tablets. Methods The separation of pulegone was carried out by using Agilent Zorbax SB C18 column(250 mm × 4.6 mm, 5 μm) with the mobile phase containing acetonitrile- 0.05% phosphoric acid aqueous with gradient elution. The detective wavelength was set at 256 nm. The flow rate was 1.0 mL/min with injection volume of 10 μL. Results The linearity range of pulegone was 0.442 3 — 1 105.8 ng(r=0.999 9), and the average recovery was 97.59% with RSD value of 1.54%(n=6). Conclusion The method is simple and accurate with good recovery and repeatability, and suitable for the quality control of Liujing Toutong Tablets.
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