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Mechanism Research of ALA-PDT on Resistant Leukemia
Author(s): Han Xiaofeng, Huang Honghui, Ni Beiwen, Zhong Lu, Zhong Jihua, Chen Fangyuan, Department of Hematology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Pages: 76-
80
Year: 2014
Issue:
1
Journal: Applied Laser
Keyword: 5-ALA; photodynamic therapy; HL-60/ADR; cell apoptosis; mitochondrial; bcl-2; multiresistant protein;
Abstract: Objective:To investigate the mechanisms of ALA-PDT-induced cell death in resistant HL-60/ADR leukemia cell line and to determine the inhibition of cell proliferation after ALA-PDT on resistant primary leukemia cells.Methods:Using HL-60/ADR cell line as a model,the cells were divided into four groups,only ALA group,only PDT group,ALA+PDT group and control group.Flow cytometry analysis was used to detect variations in Mitochondrial Membrane Potential(MMP)by using the lipophilic cation 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazol-carbocyanine iodide(JC-1).Real time-PCR were used to evaluate the mRNA expression of Bcl2and MRP.Results:The fraction of cells with the disrupted membrane potential was significantly higher after ALA combined with irradiation with time-dependence.The results revealed that ALA-PDT down-regulated Bcl2and MRP expression in HL-60/ADR cell line.ALA-PDT can inhibit the proliferation of leukemia primary cells.Conclusions:ALA-PDT may alter mitochondrial function of the cells,resulting in loss of MMP and down regulated Bcl2 and MRP gene expression.
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