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Technology Research on Juglone Extraction from Walnut Peel by Enzyme Assisted Ultrasonic Microwave
Pages: 73-76
Year: Issue:  1
Journal: Amino Acids and Biotic Resources

Keyword:  walnut peelenzymatic hydrolysisultrasonic microwave extraction instrumentjuglone;
Abstract: Based on the optimization of extraction conditions of enzyme assisted ultrasonic microwave,preparation of juglone from walnut peel by silica gel chromatography was studied,and the purity and concentration were testde. The results showed that the best hydrolysis conditions were equal mixture of 1% cellulase and 1% pectinase 400 μL,solid-liquid ratio of 1∶20,temperature of 55 ℃,pH of 6.2,time of 15 h. The optimum extraction conditions:chloroform used as extraction agent and solid-liquid ratio of 1∶20,extraction of 15 min by ultrasonic microwave,temperature of 55,60,65 ℃,ultrasonic power of 800,900,1000 W,microwave power of 200,250,300 W,ultrasonic frequency 25 kHz,15∶10 of mode,motor speed of 900 r·min-1. Dry sample was loaded onto a silica gel column,and chloroform and petroleum ether were used to elute juglone product. Photometer measured juglone average content of 0.49%,and purity of 81.7%.
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