Author(s): ZENG Ling, WANG Hai-kuan
Pages: 25-27,28
Year: 2014 Issue:  5
Journal: Chinese Manipulation & Qigong Therapy
Keyword:  xingnaojing injection; PC12 cells; apoptosis; GABAA receptor; correlation;
Abstract: Objective:To elucidate the role of GABAA receptor in the protective effect of xingnaojing injection on neurons apoptosis. Methods:The PC12 apoptosis model was established by serum starvation for 3 days. The cells were randomly classified into four groups: control group, model group, xingnaojing group (XNJ group), GABAA receptor antagonist Group (BIC group), xingnaojin and GABAA receptor antagonist Group (XNJ+BIC group). The control group only cultured with 10%FBS of normal medium;model group treated with serum-free culturel;XNJ group joined for 1.5μL/mL xingnaojing injection after the serum-free culturel. BIC Group joined 200μmoL/mL bicuculline;XNJ+BIC group joined 1.5μL/mL xing-naojing injection and 200μmoL/mL bicuculline. Each group has 5 double wells. Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the expression of GABAA receptor protein. Results:XNJ+BIC group compared with model group, the apoptosis is significantly decreased (P<0.05). The expression of GABAA is significantly decreased by the combination of xingnao-jing injection and GABAA receptor antagonist bicuculline. Conclusion:The mechanisms of xingnaojing injection protection against cell apoptosis are related to the anti-apoptosis effect of GABAA receptor.