Author(s): ZHU Ming-Xia, WANG Jing, KE Xiao-Yan, Department of Hematology, Peking University Third Hospital
Pages: 148-153
Year: 2014 Issue:  1
Journal: Journal of Experimental Hematology
Keyword:  human embryonic stem cell; hematopoietic progenitor; extracellular matrix; direct adherent culture;
Abstract: This study was purposed to establish a new inducing system for differentiation of human embryonic stem cells into hematopoietic progenitor cells and to explore the effect of different extracellular matrices(DEM) on production of hematopoietic cells.The 3 kinds of extracellular matrices——matrigel,fibronectin and IV type collagen(collagen IV)were chosen to package cultured plates,the direct adherent culture on extracellular mafrixs was used,and the hematopoietic grow th factors were added into cultured plates to induce the differentiation of human embryonic stem cells into hematopoietic progenitor cells.The hematopoietic colony forming unit assay was used to determine the yielded colony forming cells,the flow cytometry and real-time quantitative PCR were used to detect the expression of markers specific to hematopoiesis and the effect of 3 extracellular matrices on production of hematopoietic progenior cells was compared.The results showed that after being induced for 14 days,the total yield of colony forming cells,the ratio of CD34+cells and the expression level of SCL and CD34 on collogen IV were significantly higher than those on matrigel and fibronectin groups(P < 0.05).It is concluded that human embryonic stem cells can efficiently differentiate into hematopoietic progenitor cells by direct adherent culture on extracellular matrices,and the collagen IV can improve the hematopoietic differentiation of human embryonic stem cells.