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Effects of Melittin on Expression of HIF-1α and Vascular Endothelial Growth Factors of Saos-2 Cell Line
Author(s): 
Pages: 63-66
Year: Issue:  6
Journal: Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai

Keyword:  MelittinosteosarcomaSaos-2 cell linehypoxia-inducible factor-1α(HIF-1α)vascular endothelial growth factor(VEGF);
Abstract: Objective: To investigate the effects of melittin on expression of HIF-1α and vascular endothelial growth factors( VEGF) of Saos-2 cell line,and to explore its mechanism. Methods: We set up blank control group,hypoxia group and melittin groups of different concentrations( 2 μg / ml,4 μg / ml,8 μg / ml). The HIF-1α expression was detected by WesternBlot and changes of transcriptional levels of HIF-1α and VEGF mRNA were detected by RT-PCR. Results: Compared with blank control group,the expressions of HIF-1α and VEGF mRNA were increased in hypoxia group markedly( P < 0. 05); the HIF-1α expressions in Melittin groups were lower than that of the hypoxia group( P < 0. 05),with dose-dependent manner; the VEGF mRNA levels in melittin groups were lower than that of the hypoxia group( P < 0. 05),with dose-dependent manner. Conclusion: Simulated hypoxia condition can induce the activity of Saos-2 pathway in Saos-2 cell line. Melittin can inhibit the the activity of Saos-2 pathway in Saos-2 cell line by inhibiting the HIF-1α expression and influencing VEGF mRNA expression. So we think that melittin may inhibit osteosarcoma angiogenesis by this way to achieve anti-tumor effect.
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