Changes of Cell Structure and the Surface Glycoproteins in the Leaves of Wheat under Cold Stress at Tillering Stage
Author(s): XU Wen, YANG Jing, ZHEN Mingming, DENG Lele, GUO Wenshan, ZHU Xinkai, LI Chunyan, Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Crop Physiology, Ecology and Cultivation in Middle and Lower Reaches of Yangtse River of Ministry of Agriculture, Yangzhou University, Agricultural Technology Promotion Center of Agriculture and Forestry Bureau
Pages: 403-411
Year: 2014 Issue: 3
Journal: Journal of Triticeae Crops
Keyword: Wheat; Low temperature; Cell structure; Glycoprotein; Cold resistance;
Abstract: Cytochemical changes of glycoproteins localized at the cell surface and the cell structure in the leaves of wheat were observed by electron microscopy using Yangmai 16 and Xumai 30 as materials treated under low temperature to explore the their relations with cold tolerance.The results showed that the freezing-injured rate increased with lower temperature and longer duration of stress.Yangmai16 had weaker resistance to low temperature than Xumai 30.Plasmolysis occurred in the cells when Yangmai 16 was treated with-6℃ and-10℃ for 24h,while Xumai 30 did not.Under longer duration of lower temperature,the chloroplasts became swollen and round,the arrangement directions of thylakoid changed and cell cavitation was observed,part of the organelles disappeared,even noth-ing could be observed in the cells.At the beginning of low temperature treatment,a few glycoproteins were attached to the diachyma cytoderm,which appeared loose filamentous structure.The keeping time of the glycoproteins attaching the cell surface of Xumai 30 was longer than that of Yangmai 16.The longer the low temperature was,the more serious the cold damage on cells of both cultivar plants was.The cell surface glycoproteins appeared to move into the intercellular spaces or inside the cell.The different changes of cell structure and surface glycoproteins were one of the physiological reasons of wheat cultivars with different cold hardiness.
Pages: 403-411
Year: 2014 Issue: 3
Journal: Journal of Triticeae Crops
Keyword: Wheat; Low temperature; Cell structure; Glycoprotein; Cold resistance;
Abstract: Cytochemical changes of glycoproteins localized at the cell surface and the cell structure in the leaves of wheat were observed by electron microscopy using Yangmai 16 and Xumai 30 as materials treated under low temperature to explore the their relations with cold tolerance.The results showed that the freezing-injured rate increased with lower temperature and longer duration of stress.Yangmai16 had weaker resistance to low temperature than Xumai 30.Plasmolysis occurred in the cells when Yangmai 16 was treated with-6℃ and-10℃ for 24h,while Xumai 30 did not.Under longer duration of lower temperature,the chloroplasts became swollen and round,the arrangement directions of thylakoid changed and cell cavitation was observed,part of the organelles disappeared,even noth-ing could be observed in the cells.At the beginning of low temperature treatment,a few glycoproteins were attached to the diachyma cytoderm,which appeared loose filamentous structure.The keeping time of the glycoproteins attaching the cell surface of Xumai 30 was longer than that of Yangmai 16.The longer the low temperature was,the more serious the cold damage on cells of both cultivar plants was.The cell surface glycoproteins appeared to move into the intercellular spaces or inside the cell.The different changes of cell structure and surface glycoproteins were one of the physiological reasons of wheat cultivars with different cold hardiness.
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