Author(s): WANG Yu-hua, FU Li-jia, XU Lian-qin, YANG Bao-feng, Daqing Oilfield General Hospital
Pages: 70-72
Year: 2014 Issue:  2
Journal: Information on Traditional Chinese Medicine
Keyword:  HPLC-ELSD; Notoginsenoside R1; Ginsenoside Rg1; Ginsenoside Rb1; Fufang Xueshuantong capsules;
Abstract: Objective: To establish simultaneous assay of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Fufang Xueshuantong capsules by HPLC- ELSD. Methods: The column was packed with a DiamonsilC18( 4. 6mm × 250mm,5μm) stationary phase. The mobile phase consisted of acetonitrile( A)- water( B),eluted in gradient mode( 0 min→5min→20min,A: 20%→25%→45%) with flow rate of 1. 0mL/min. Column temperature was set at 25℃. The temperature of driftube was 70℃ and the air flow rate was 2. 0L / min. Results: The linear ranges of notoginsenoside R1,ginsenoside Rg1and Rb1 were 0. 3 ~ 1. 5μg( r = 0. 9994),1. 5 ~ 7. 5μg( r = 0. 9995) and 1. 5 ~ 7. 5μg( r = 0. 9999) respectively; the average recoveries( n = 6) were 98. 8%,98. 2% and 97. 6%,with the RSD of 1. 1%,1. 8% and 1. 3% respectively. Conclusion: The method is rapid,simple and accurate. It can be used for quality evaluation of Fufang Xueshuantong capsules.