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MOLECULAR CLONING AND EXPRESSION ANALYSIS OF PROTEIN DISULFIDE ISOMERASE GENE IN THE MUD CRAB(SCYLLA PARAMAMOSAIN)
Author(s): 
Pages: 1512-1518
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  protein disulfide isomeraseScylla paramamosainovarian developmentreal-time quantitative RT-PCRimmunochemistry;
Abstract: Protein disulfide isomerase(PDI) is a multifunctional protein in all eukaryotic cells for assisting many protein maturation processes within the endoplasmic reticulum(ER). We cloned and characterized PDI gene from mud crab Scylla paramamosain, and studied its localization and developmental expression. The full length of cDNA was 2016bp long with an ORF of 1452bp encoding a putative peptide of 483 amino acids(aa). The deduced aa sequence of Sp-PDI showed high similarity to related sequences of other species. Molecular analysis was carried out by examining gene expression via real-time quantitative RT-PCR. In the crabs of same developmental stage and size, Sp-PDI transcripts occurred in all the various tissues, including ovary, hepatopancreas, hemocyte, muscle, heart, thoracic ganglia, cerebral ganglia, stomach, gill, eyestalk, and epidermis. In the ovaries of different stages, Sp-PDI expression increased gradually until it become matured and then decreased at last. By means of immunochemistry, PDI reactivity was found in the oocyte of S. paramamosain. These results suggest that Sp-PDI is implicated during the growth and ovarian development.
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