CORRELATION OF EST-SSR MARKERS WITH GROWTH TRAITS IN CHINESE SOFT-SHELLED TURTLES(PELODISCUS SINENSIS)
Author(s): ZHANG Qun-Ying, HUANG He-Zhong, YUAN Wen-Cheng, LI Wen-Long, YONG Fu-He, XU Xue-Ying, ZHANG Ping, School of Medicine and Life Sciences, Medical College of Soochow University, Fisheries Research Institute of Soochow University, Mashe Special Aquaculture Farms in Xukou Town, Wuzhong District, Suzhou City, Fisheries Technology Extension Station of Suzhou City, Fisheries Technology Guide Station of Suining County in Xuzhou City
Pages: 1654-1660
Year: 2013 Issue: 6
Journal: Oceanologia et Limnologia Sinica
Keyword: Pelodiscus sinensis; EST-SSR marker; growth trait; correlation analysis;
Abstract: 15 polymorphic microsatellites were developed from the transcriptome sequencing EST database of Chinese soft-shelled turtles(Pelodiscus sinensis) to analyze the genetic diversity and linkage of 60 individuals. The result indicate that: 15 EST-SSR markers obtained amplified products successfully in a high polymorphism level; the number of mean valid alleles was 2.7633; the value of mean expected heterozygosity was 0.5961; and the mean polymorphism information content(PIC) was 0.539177. The linkage analysis showed the locus C8387 had a significant impact on body weight, body length, carapace length and a extremely significant difference in side width(P<0.01), and CC at C8387 was a favorable genotype for the four growth traits while AB was a disadvantage genotype; locus C211 showed an extremely significant impact on body weight, carapace length(P<0.01) and a significant impact on the side width(P<0.05), among them allele C was negatively associated with the body weight and genotype CC was disadvantage genotype of carapace length and side width, while BB and AB were favorable genotypes of side width and body weight respectively; locus C5670 had an extremely significant impact on body weight(P<0.01) and a significant impact on carapace length(P<0.05), in which BB was the dominant genotype of body weight and carapace length while the AA was the negative genotype; the locus C1312 and C13038 had a extremely significant correlation of body weight, carapace length, respectively(P<0.01), in which BC of locus C1312 and BB of C13038 was dominant genotype of body weight and carapace length, respectively.
Pages: 1654-1660
Year: 2013 Issue: 6
Journal: Oceanologia et Limnologia Sinica
Keyword: Pelodiscus sinensis; EST-SSR marker; growth trait; correlation analysis;
Abstract: 15 polymorphic microsatellites were developed from the transcriptome sequencing EST database of Chinese soft-shelled turtles(Pelodiscus sinensis) to analyze the genetic diversity and linkage of 60 individuals. The result indicate that: 15 EST-SSR markers obtained amplified products successfully in a high polymorphism level; the number of mean valid alleles was 2.7633; the value of mean expected heterozygosity was 0.5961; and the mean polymorphism information content(PIC) was 0.539177. The linkage analysis showed the locus C8387 had a significant impact on body weight, body length, carapace length and a extremely significant difference in side width(P<0.01), and CC at C8387 was a favorable genotype for the four growth traits while AB was a disadvantage genotype; locus C211 showed an extremely significant impact on body weight, carapace length(P<0.01) and a significant impact on the side width(P<0.05), among them allele C was negatively associated with the body weight and genotype CC was disadvantage genotype of carapace length and side width, while BB and AB were favorable genotypes of side width and body weight respectively; locus C5670 had an extremely significant impact on body weight(P<0.01) and a significant impact on carapace length(P<0.05), in which BB was the dominant genotype of body weight and carapace length while the AA was the negative genotype; the locus C1312 and C13038 had a extremely significant correlation of body weight, carapace length, respectively(P<0.01), in which BC of locus C1312 and BB of C13038 was dominant genotype of body weight and carapace length, respectively.
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