Cloning and Analysis of the Myostatin Introns and Promoter in Elopichthys bambusa
Author(s): YU Jian-feng, GU Zhi-liang, SHAO Fang, FENG Xiao-xia
Pages: 17-22
Year: 2014 Issue: 2
Journal: Journal of Changshu Institute of Technology
Keyword: Elopichthys bambusa; myostatin; intron; promoter;
Abstract: The objective of this study is to clone the sequences of myostatin gene introns and its promoter in Elopichthys bambusa. PCR and Genome Walking were used to acquire two introns and promoter of the mostatin gene. Two introns and a partial sequence of the myostatin promoter were cloned, of which the lengths were 789 bp, 987 bp and 622 bp, respectively. Bioinformatics analysis showed that the sequences of the two myostatin in-trons of Elopichthys bambusa had higher similarity to the myostatin introns of other cyprinidae fish (intron 1:60.42%-67.21%, intron 2: 40.00%-51.21%) than the other animals′. The nucleotide sequence analysis showed that the partial sequence of the myostatin promoter contained several potential regulatory factors binding sites, of which are two TBP, two CTF, three Sp1, ten C/EBP, four Oct1, two AP1, one GATA, three HNF, one NF-κB, one CREB, three E-box and so on.
Pages: 17-22
Year: 2014 Issue: 2
Journal: Journal of Changshu Institute of Technology
Keyword: Elopichthys bambusa; myostatin; intron; promoter;
Abstract: The objective of this study is to clone the sequences of myostatin gene introns and its promoter in Elopichthys bambusa. PCR and Genome Walking were used to acquire two introns and promoter of the mostatin gene. Two introns and a partial sequence of the myostatin promoter were cloned, of which the lengths were 789 bp, 987 bp and 622 bp, respectively. Bioinformatics analysis showed that the sequences of the two myostatin in-trons of Elopichthys bambusa had higher similarity to the myostatin introns of other cyprinidae fish (intron 1:60.42%-67.21%, intron 2: 40.00%-51.21%) than the other animals′. The nucleotide sequence analysis showed that the partial sequence of the myostatin promoter contained several potential regulatory factors binding sites, of which are two TBP, two CTF, three Sp1, ten C/EBP, four Oct1, two AP1, one GATA, three HNF, one NF-κB, one CREB, three E-box and so on.
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