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Knock out deletion and functional analysis of glucose transportation system in Xanthomonas campestris pv.campestris
Author(s): MA Chen, LIANG Ru-bing, LIU Jian-hua
Pages: 100-
104
Year: 2014
Issue:
1
Journal: Acta Agriculturae Zhejiangensis
Keyword: Xanthomonas campestris pv.campestris; glucose transporter; gene deletion; function analysis;
Abstract: Three glucose transporters and two membrane-bound glucose dehydrogenases were knocked out in the phy-topathogenic bacterium Xanthomonas campestris pv.campestris 8004.The five mutants and Xcc8004 had little differ-ence in their basic metabolisms , such as cell growth curve , extra-cellular polysaccharide production and cellulases activities when cultured in nutrition-rich medium.However, the deletion of sodium/glucose transporter gene (XC_2460) influenced the cell growth when glucose was used as carbon source ,but its extra-cellular glucose yield in car-boxymethyl cellulose degradation test was about 1.67-fold higher than those of other strains .These results demonstra-ted the hypothesis that the extracellular glucose concentration from cellulose could be increased significantly by block of glucose influx transport system without affecting basic cell metabolism is feasible .
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