Author(s): LI Dan, CHU Yang, Dept. of Pharmacy, Fengtian Hospital of Shenyang Medical College, Dept. of Pharmacy, The First Affiliated Hospital of China Medical University
Pages: 51-53
Year: 2014 Issue:  1
Journal: China Pharmacy
Keyword:  Sodium hyaluronate; Microspheres; Preparation technology; Orthogonal test;
Abstract: OBJECTIVE:To prepare Sodium hyaluronate-loaded microspheres,and to provide reference for sustained-release preparation. METHODS:Sodium hyaluronate-loaded microspheres were prepared by emulsion-cross linking method. The single factor test was performed for prescription factor and technology factor. The preparation technology of microspheres was optimized by orthogonal test with encapsulation efficiency(EE)as index using the concentration of sodium alginate(A),ratio of sodium hyaluronate to sodium alginate(B),crosslinking time(C)and dispersion speed(D)as factors. The investigating particle size and EE of microspheres verification test was carried out by particle size and EE of microspheres. RESULTS:The optimal technology was as follows:A of 2%,B of 1 ∶3,C of 90 min and D of 200 r/min. The EE of the microspheres was(58.92±3.06)% with mean diameter of(107.8±5.51)μm. CONCLUSIONS:The emulsion-cross linking method is simple,feasible and lays the foundation for the preparation of Sodium hyaluronate-loaded sustained-release preparation.