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Issue:
Isolation and catalytic characteristics of ethylene glycol-oxidizing strain
Author(s):
HE Yucai
,
XU Xiaofeng
,
PAN Xuehe
,
WU Miaoxin
Pages:
29
-
33
Year:
2013
Issue:
6
Journal:
Chinese Journal of Bioprocess Engineering
Keyword:
oxidation
;
ethylene glycol
;
glycolic acid
;
Abstract:
利用富集培养技术从土壤中筛选获得1株高活性二醇氧化活性菌株Brevibacterium sp.CCZU12-1.以Brevibacterium sp.CCZU12-1静息细胞为催化剂,最适催化反应温度、反应pH和金属离子添加量分别为30℃、6.5和Mn2+0.1 mmol/L.在最佳条件下,转化200 mmol/L乙二醇24 h,羟基乙酸的产率为94.6%,分批补料乙二醇5批,羟基乙酸的累积浓度为972 mmol/L.
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