Effect of metformin on insulin resistant adipocyte
Author(s): WU Naijun, JIN Xiuping, SHENG Jiazuo, SHEN Chunzuo, WEI Jianfen, WANG Ying, CHEN Dong, MENG Qingling, JIAO Wenye
Pages: 66-69
Year: 2014 Issue: 1
Journal: China Medicine
Keyword: Insulin resistance; Metformin; 3T3-L1 adipocyte; Adenosine monophosphate activated protein-activated protein kinase;
Abstract: Objective To study the effects of metformin on insulin resistant 3T3-L1 adipocyte.Methods 3T3-L1 adipocyte was chosen as our experimental object.When the resistance model was established,the adipocyte was divided into blank group,model group,0.01 mmol/L metformin group,0.10 mmol/L metformin group,0.50 mmol/L metformin group and 1.00 mmol/L metformin group.After metformin was intervented for 48 hours,the level of glucose in the medium and the mRNA expression of adenosine monophos-phate activated protein kinase (AMPK) were examined.Results The absorbance level of model group (2.539 0 ± 0.002 4) was significant higher than that of the blank group (1.477 8 ± 0.003 3) (P ＜ 0.05).The absorbance level in 1.00,0.50,0.10,0.01 mmol/L metformin group(1.740 0 ±0.002 4,1.741 8 ±0.002 8,2.178 5 ±0.001 9,2.369 3 ± 0.002 9) were lower than that in the model group (0.120 ± 0.006) (P ＜ 0.05).The expression of AMPK mRNA in model group (0.120 ± 0.006)was significant lower than that in the blank group(1.000 ± 0.000)(P ＜ 0.05).The expression of AMPK mRNA in each metformin group (0.24 ± 0.006,0.53 ± 0.035,0.85 ± 0.020,0.89 ± 0.041) was significant higher than that in the model group (0.120 ± 0.006) (P ＜ 0.05).Conclusion Metformin can improve the glucose intake in insulin resistant 3T3-L1 adipocyte.Metformin may increase the glucose intake in insulin resistant 3T3-L1 adipocyte by increasing the expression of AMPK mRNA.
Pages: 66-69
Year: 2014 Issue: 1
Journal: China Medicine
Keyword: Insulin resistance; Metformin; 3T3-L1 adipocyte; Adenosine monophosphate activated protein-activated protein kinase;
Abstract: Objective To study the effects of metformin on insulin resistant 3T3-L1 adipocyte.Methods 3T3-L1 adipocyte was chosen as our experimental object.When the resistance model was established,the adipocyte was divided into blank group,model group,0.01 mmol/L metformin group,0.10 mmol/L metformin group,0.50 mmol/L metformin group and 1.00 mmol/L metformin group.After metformin was intervented for 48 hours,the level of glucose in the medium and the mRNA expression of adenosine monophos-phate activated protein kinase (AMPK) were examined.Results The absorbance level of model group (2.539 0 ± 0.002 4) was significant higher than that of the blank group (1.477 8 ± 0.003 3) (P ＜ 0.05).The absorbance level in 1.00,0.50,0.10,0.01 mmol/L metformin group(1.740 0 ±0.002 4,1.741 8 ±0.002 8,2.178 5 ±0.001 9,2.369 3 ± 0.002 9) were lower than that in the model group (0.120 ± 0.006) (P ＜ 0.05).The expression of AMPK mRNA in model group (0.120 ± 0.006)was significant lower than that in the blank group(1.000 ± 0.000)(P ＜ 0.05).The expression of AMPK mRNA in each metformin group (0.24 ± 0.006,0.53 ± 0.035,0.85 ± 0.020,0.89 ± 0.041) was significant higher than that in the model group (0.120 ± 0.006) (P ＜ 0.05).Conclusion Metformin can improve the glucose intake in insulin resistant 3T3-L1 adipocyte.Metformin may increase the glucose intake in insulin resistant 3T3-L1 adipocyte by increasing the expression of AMPK mRNA.
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