Polyploid Induction and Identification of Shandong Platycodon grandiflorum
Author(s): Zhu Jingbin, Shan Chenggang, Wang Weiting, Wang Zhifen
Pages: 32-34
Year: 2013 Issue: 10
Journal: Shandong Agricultural Sciences
Keyword: Platycodon grandiflorum; Cochicine; Dimethyl sulfoxide; Polyploid induction;
Abstract: 在离体培养条件下,比较了单用不同浓度秋水仙素和0.2％秋水仙素与不同浓度二甲基亚砜(DMSO)混用对山东桔梗愈伤组织诱导多倍体的效果.结果表明:用0.2％秋水仙素和2％ DMSO浸泡山东桔梗愈伤组织16 h,多倍体诱导率最高,达43.3％.桔梗多倍体植株叶颜色深、有皱褶,叶片大且厚,茎秆粗壮,节间距长.经显微鉴定,多倍体叶气孔增大,但单位面积气孔数目减少.
Pages: 32-34
Year: 2013 Issue: 10
Journal: Shandong Agricultural Sciences
Keyword: Platycodon grandiflorum; Cochicine; Dimethyl sulfoxide; Polyploid induction;
Abstract: 在离体培养条件下,比较了单用不同浓度秋水仙素和0.2％秋水仙素与不同浓度二甲基亚砜(DMSO)混用对山东桔梗愈伤组织诱导多倍体的效果.结果表明:用0.2％秋水仙素和2％ DMSO浸泡山东桔梗愈伤组织16 h,多倍体诱导率最高,达43.3％.桔梗多倍体植株叶颜色深、有皱褶,叶片大且厚,茎秆粗壮,节间距长.经显微鉴定,多倍体叶气孔增大,但单位面积气孔数目减少.
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