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Optimization of Fermentation Process for Chlamydospores of Trichoderma asperellum by Response Surface Methodology
Pages: 97-104
Year: Issue:  12
Journal: China Biotechnology

Keyword:  BBD design of response surface methodologyTrichoderma asperellumChlamydospores Fermentation processOptimize;
Abstract: The response surface methodology(Box-Benhnken Design) was applied to investigate the four major factors(the concentration of soybean cake powder and maize powder used in medium,the volume of glycerol addition added to the medium,loading volume of liquid medium,initial pH value of medium) that have importent effect on the production of chlamydospores of Trichoderma asperellum ZJSX5003.By variance analysis on the experiment results,the influences of the the four factors and their interactions on the production of chlamydospores was investigated.By performing regression analysis and establishing regression equation based on statistics,the optimal fermentation condition was obtained,that as follow:the concentration of soybean cake powder and maize powder used in medium 33.25 g /L,glycerol 8.86ml/L,each 500ml bottles contains 99.35 ml liquid medium,initial pH 3.26.Under the optimal conditions,the yield of chlamydospore is expected to be 9.56×10~7 spores/ml,theoretically.The practical yield of chlamydospores approached 9.84×10~7spores/ml,reached97.07%of the expected value,increased by 69.07%compared with pre-optimization.In order to test its applicability,used a 10L fermentation tank under the optimal condition,the yield of chlamydospore reached up to1.75×10~8 spores/ml,which is better than shake flask fermentation.Further researches of developing microbial inoculum of Trichoderma chlamydospore could be conducted base on this research.
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