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jiang lao shi xin xiang
Author(s): 
Pages: 20
Year: Issue:  12
Journal: China Biotechnology

Keyword:  原核表达包涵体分离纯化技术蛋白质老师研讨班学员表达载体低浓度大肠杆菌;
Abstract: <正>蛋白质研讨班学员问:姜老师,您好!我是您"蛋白质分离纯化技术专题研讨班"的学员,有问题想向您请教。我在做蛋白的原核表达,用的是pkk223-3载体,表达菌株用了大肠杆菌JM109和TB1,发现表达蛋白均在包涵体中,降低IPTG浓度为0.05mM,降低诱导
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