Author(s): AN Jun-ying, LIU Ying, ZHU Wen-juan, HU Xue-qiong, YE Li-zhen, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, College of Food Science and Technology, Guangdong Ocean University
Pages: 191-195
Year: 2014 Issue:  1
Journal: Science and Technology of Food Industry
Keyword:  response surface methology; bacteriocin-like substance; fermentation medium;
Abstract: The fermentation medium of Bacillus amyloliquefacien ZJHD- 06 isolated from intestine of Epinephelus areolatus was optimized for a bacteriocin-like substance( BLS) production.Bacteriostatic circle diameter was used as evaluation index against L.monocytogenes ATCC 35152.Firstly,the notable factors were screened by Plackett- Burman experiment design.Then,the path of steepest ascent was undertaken to approach the optimal region of the significant factors.Finally,Box-Behnken design was adopted to identify optimal proportion.The results showed the optimal proportions were glucose 4.80%,peptone 0.47%,yeast extract 0.44%,NaCl 1.0%,K 2 HPO 4 0.01% and MgSO 4 ·7H 2 O 0.03%. The bacteriostatic circle diameter of the fermentation supernatant was increased by 27.8% using the optimal proportions.