Structure, DNA Binding Studies and Cytotoxicity of Complex [Pd(phen)(L-asp)]·3H2O
Author(s): GAO Enjun, LIU Lei, SHI Chunyue, YIN Hongxi, ZHU Mingchang, WU Qiong, LIU Qitao
Pages: 1061-1066
Year: 2009 Issue: 6
Journal: Chinese Journal of Chemistry
Keyword: palladium complex; cytotoxic activity; DNA-binding;
Abstract: The pal ladium(Ⅱ) complex of [Pd(phen)(L-asp)].3H2O (phen= 1,10-phenanthroline, H2L-asp=L-aspartic acid) has been synthesized from a solution reaction and analyzed by elemental analyses, 1H NMR and IR spectra. More-over, the complex has been structurally characterized by single-crystal X-ray diffractometry. The cytotoxicity assay of the complex and cis-DDP as reference substance against three different cancer cell lines (Hela, Hep-G2 and KB)has been conducted. The results show that the Pd complex exhibits higher cytotoxicity against Hela system. The study on the interaction of the Pd complex with fish sperm DNA (FS-DNA) has been performed with diverse spec-troscopic techniques, showing that the complex is bound to the fish sperm DNA via an intercalative mode. Gel elec-trophoresis assay demonstrates the ability of the complex to cleave the pBR 322 plasmid DNA.
Pages: 1061-1066
Year: 2009 Issue: 6
Journal: Chinese Journal of Chemistry
Keyword: palladium complex; cytotoxic activity; DNA-binding;
Abstract: The pal ladium(Ⅱ) complex of [Pd(phen)(L-asp)].3H2O (phen= 1,10-phenanthroline, H2L-asp=L-aspartic acid) has been synthesized from a solution reaction and analyzed by elemental analyses, 1H NMR and IR spectra. More-over, the complex has been structurally characterized by single-crystal X-ray diffractometry. The cytotoxicity assay of the complex and cis-DDP as reference substance against three different cancer cell lines (Hela, Hep-G2 and KB)has been conducted. The results show that the Pd complex exhibits higher cytotoxicity against Hela system. The study on the interaction of the Pd complex with fish sperm DNA (FS-DNA) has been performed with diverse spec-troscopic techniques, showing that the complex is bound to the fish sperm DNA via an intercalative mode. Gel elec-trophoresis assay demonstrates the ability of the complex to cleave the pBR 322 plasmid DNA.
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