Protective Effects of Ethanol Extract of Pueraria lobata against Blood Lipid and Blood Vessel in Hyperglycemia Model Rats
Author(s): YANG Zhi-yong, The Affiliated Hospital of Chengdu University
Pages: 4063-4065
Year: 2013 Issue: 43
Journal: China Pharmacy
Keyword: Pueraria lobata; Hyperglycemia; Rats; NO; ET-1;
Abstract: OBJECTIVE:To study the protective effects of ethanol extract of Pueraria lobata against blood lipid and blood vessel in hyperglycemia model rats. METHODS:Experiment is divided into normal control(constant volume physiological saline) group,model(constant volume physiological saline)group and P. lobata alcohol extraction high-dose,medium-dose and low dose(300,150,75 mg/kg)group. Rats were intragastrically administered,once a day for two months. High sugar and high fat diet combined with disposable intraperitoneal injection of STZ were adopted to induce hyperglycemia model. The body weight and daily water quantity were determined,and automatic biochemistry analyzer was used to determine the contents of FBG,TC,TG, HDL-C and LDL-C. Nitrate enzyme reduction method was used to determine NO content;double antibody sandwich solid-phase enzyme immunoassay was adopted to determine the levels of ET-1. RESULTS:Compared with normal control group,body weight and the contents of HDL-C and NO were decreased significantly in model group,and daily water quantity,FBG,the levels of TC,TG and LDL-C and the content of ET-1 were increased significantly(P<0.01). Compared with model group,body weight, the contents of HDL-C and NO were increased significantly in ethanol extract of P. lobata high-dose and medium-dose groups,and daily water quantity,FBG,the contents of TC,TG,LDL-C and ET-1 were decreased significantly(P<0.01 or P<0.05). CONCLUSIONS:The ethanol extract of P. lobata can improve blood lipid and blood vessel in hyperglycemia model rats. Its mechanism may be associated with regulating blood lipid and maintaining dynamic stability of vascular active substances.
Pages: 4063-4065
Year: 2013 Issue: 43
Journal: China Pharmacy
Keyword: Pueraria lobata; Hyperglycemia; Rats; NO; ET-1;
Abstract: OBJECTIVE:To study the protective effects of ethanol extract of Pueraria lobata against blood lipid and blood vessel in hyperglycemia model rats. METHODS:Experiment is divided into normal control(constant volume physiological saline) group,model(constant volume physiological saline)group and P. lobata alcohol extraction high-dose,medium-dose and low dose(300,150,75 mg/kg)group. Rats were intragastrically administered,once a day for two months. High sugar and high fat diet combined with disposable intraperitoneal injection of STZ were adopted to induce hyperglycemia model. The body weight and daily water quantity were determined,and automatic biochemistry analyzer was used to determine the contents of FBG,TC,TG, HDL-C and LDL-C. Nitrate enzyme reduction method was used to determine NO content;double antibody sandwich solid-phase enzyme immunoassay was adopted to determine the levels of ET-1. RESULTS:Compared with normal control group,body weight and the contents of HDL-C and NO were decreased significantly in model group,and daily water quantity,FBG,the levels of TC,TG and LDL-C and the content of ET-1 were increased significantly(P<0.01). Compared with model group,body weight, the contents of HDL-C and NO were increased significantly in ethanol extract of P. lobata high-dose and medium-dose groups,and daily water quantity,FBG,the contents of TC,TG,LDL-C and ET-1 were decreased significantly(P<0.01 or P<0.05). CONCLUSIONS:The ethanol extract of P. lobata can improve blood lipid and blood vessel in hyperglycemia model rats. Its mechanism may be associated with regulating blood lipid and maintaining dynamic stability of vascular active substances.
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