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SCREENING,EXPRESSION AND FUNCTION OF smu-1 GENES FROM MACROBRACHIUM ROSENBERGII
Author(s): 
Pages: 1008-1015
Year: Issue:  4
Journal: Oceanologia et Limnologia Sinica

Keyword:  Macrobrachium rosenbergiismu-1 geneWD-40 repeat protein familyheat shock;
Abstract: Using subtractive hybridization library of central nervous system from Macrobrachium rosenbergii,smu-1gene was screened.The amino acids sequences of smu-1 had high homology with other species,and the protein contained six WD regions at C-terminus of WD40-repeat motif,belonging to WD40-repeat protein family.Using the real-time PCR technology,we found that the expression of smu-1 gene up-regulated significantly under heat or cold shock and mental stress(P<0.05,P<0.01)compared with that of control group.At high and low osmosis conditions,the smu-1 expression showed down-regulated tendency(P<0.05).In addition,smu-1 gene was interference by injecting smu-1 double-stranded RNA in vivo.The results show that the survival rate of M.rosenbergii decreased obviously under heat shock.This study may provide a theoretical basis for screening the heat-resistant M.rosenbergii,and reduce economic losses in shrimp farming.
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