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THE GENE CLONING OF CYP19a AND ITS EXPRESSION CHARACTERIZATION ANALYSIS IN NAVODON SEPTENTRIONALIS
Author(s): 
Pages: 1086-1094
Year: Issue:  4
Journal: Oceanologia et Limnologia Sinica

Keyword:  Navodon septentrionalisP450 aromatasegene cloningmRNA expression;
Abstract: Cytochrome P450s aromatizing enzyme is the key and rate-limiting enzyme to produce estradiol(E2).There are two different genes(CYP19a and CYP19b)encoding P450 aromatizing enzyme in teleost,and are both expressed in different tissues.The complete cDNA sequence of CYP19 in Navodon septentrionalis was obtained by the techniques of RT-PCR and rapid amplification of cDNA ends(RACE).The result of multiple amino acids alignment show that there was an I-coiled coil region,an Ozol’s peptide-binding region,a heme binding region,and a aromatizing enzyme specific binding region.The CYP19a mRNA expression in different tissues of the adult fish were analyzed by semi-quantitative PCR technique and the result suggest that it was expressed in ovary only.The result of CYP19a expression pattern in the ovary at different developmental stages show the highest expression before ovulation and lowest a after ovulation.
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