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gata-4 ji yin zeng jia gu sui jian chong zhi gan xi bao kang que yang neng li de tan tao
Author(s): 
Pages: 6-9
Year: Issue:  7
Journal: Journal of Shandong University(Health Sciences)

Keyword:  GATA-4骨髓间充质干细胞低氧环境MSCs移植缺血性心肌病;
Abstract: 目的探讨GATA-4基因对骨髓间充质干细胞(MSCs)在低氧环境下存活能力的影响。方法将GATA-4基因转染大鼠MSCs,以转染空质粒的MSCs做对照,通过蛋白印迹法检测GATA-4转染效果。模拟体内心肌梗死氧化应激损伤,在低氧环境下培养48 h,观察MSCs形态变化。通过MTT细胞增殖测定、膜联蛋白V-PE凋亡检测和乳酸脱氢酶(LDH)释放评价MSCs存活能力。结果在低氧环境下,GATA-4基因修饰组与转染空质粒的对照组相比,MTT摄取增加、膜联蛋白V阳性细胞数降低、LDH释放减少,差异有统计学意义(P<0.05)。结论转录因子GATA-4可以增加MSCs抗缺氧能力,增加细胞存活;为MSCs移植治疗缺血性心肌病、增加细胞存活提供新的方法。
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