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Journal Articles
Determination of Cinobufagin and Resibufogenin in Liver Tissue by HPLC-MS/MS
Pages: 268-272
Year: Issue:  4
Journal: Journal of Forensic Medicine

Keyword:  forensic toxicologytandem mass spectrometryhigh performance liquid chromatography-tandem mass spectrometrycinobufaginresibufogenin;
Abstract: 目的 建立灵敏、准确的高效液相色谱-串联质谱法(HPLC-MS/MS)测定肝组织中华蟾酥毒基和酯蟾毒配基含量的方法. 方法 肝组织匀浆液中加入内标地塞米松,用二氯甲烷提取后挥干,残渣加入甲醇溶液溶解并上样到ProElut C18固相萃取柱上分离净化,应用HPLC-MS/MS正离子多反应监测模式测定.结果 肝组织中华蟾酥毒基和酯蟾毒配基分别在1 ~204 ng/g和1~206 ng/g范围内线性关系良好.检测限(S/N≥3)均为0.3 ng/g.基质效应为96.5%~126.7%.萃取回收率为70.0%~82.3%.日内及日间精密度均小于10%.结论 所建方法灵敏、可靠,能满足法医毒物分析的鉴定需要.
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