Tissue Culture of Seedling of Red Heart-Wood Pinus massoniana
Author(s): LIU Xihua, DENG Deliang, ZHANG Li, XING Jianhong, LIANG Yichi
Pages: 122-125
Year: 2013 Issue: 10
Journal: Journal of Henan Agricultural Sciences
Keyword: Pinus massoniana; red heart-wood; tissue culture; orthogonal experiment;
Abstract: Using orthogonal experimental design ,tissue culture of seeds of red heart-wood of Pi-nus massoniana was researched .The result showed that the germination rate of seeds of Pinus massoniana in the induction medium containing sands and aseptic water was the best with the ger-mination rate of 71 .6% .In the multiplication culture ,the medium of WPM +6-BA 2 mg/L+NAA 0 .3 mg/L+KT 0 .2 mg/L was the best with strong and high buds and the multiplication coeffi-cient of 7 .3 .In rooting culture ,the rooting rate of 1/2WPM+ABT1#0 .8 mg/L+NAA 0 .1 mg/L was the highest ,reaching 69 .7% .This study will lay a foundation for rapid propagation technique system of red heart-wood Pinus massoniana .
Pages: 122-125
Year: 2013 Issue: 10
Journal: Journal of Henan Agricultural Sciences
Keyword: Pinus massoniana; red heart-wood; tissue culture; orthogonal experiment;
Abstract: Using orthogonal experimental design ,tissue culture of seeds of red heart-wood of Pi-nus massoniana was researched .The result showed that the germination rate of seeds of Pinus massoniana in the induction medium containing sands and aseptic water was the best with the ger-mination rate of 71 .6% .In the multiplication culture ,the medium of WPM +6-BA 2 mg/L+NAA 0 .3 mg/L+KT 0 .2 mg/L was the best with strong and high buds and the multiplication coeffi-cient of 7 .3 .In rooting culture ,the rooting rate of 1/2WPM+ABT1#0 .8 mg/L+NAA 0 .1 mg/L was the highest ,reaching 69 .7% .This study will lay a foundation for rapid propagation technique system of red heart-wood Pinus massoniana .
Related Articles