Deleted in liver cancer-1 and focal adhesion kinase genes are implicated in the growth and apoptosis of malignant OVCAR-3 cells
Author(s): REN Fang, SHI Hui-rong, LIU Hui-na, ZHANG Rui-tao
Pages: 814-817
Year: 2013 Issue: 4
Journal: Chinese Journal of Experimental Surgery
Keyword: Deleted in liver cancer-1; Focal adhesion kinase; Small hairpin RNA;
Abstract: Objective To investigate the effects of deleted in liver cancer-1 (DLC-1) and focal adhesion kinase (FAK) on the growth and apoptosis of malignant OVCAR-3 cells.Methods DLC-1 expression plasmid,FAK specific small hairpin RNA (shRNA) expression plasmid,and FAK-shRNA combined DLC-1 expression plasmid were constructed and transfected into OVCAR-3 cells.Real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting were used to detect the expression of DLC-1 and FAK in OVCAR-3 cells,cell proliferation and apoptosis were examined by cell counting kit-8 (CCK-8) kit and flow cytometry,and the expression of phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) was measured by using Western blotting.Results While the DLC-1 expression increased or/and FAK knocked down,the growth of OVCAR-3 cells was obviously inhibited [(0.737 ± 0.094),P ＜0.05],but apoptosis was significantly induced [(15.21 ± 1.05) ％,P ＜ 0.05].In addition,the expression of p-ERK1/2 was notably decreased [(0.151 ±0.010),P ＜0.05].Conclusion DLC1 might be implicated in the growth and apoptosis of malignant tumor cells via its regulation on FAK and downstream ERK signaling.
Pages: 814-817
Year: 2013 Issue: 4
Journal: Chinese Journal of Experimental Surgery
Keyword: Deleted in liver cancer-1; Focal adhesion kinase; Small hairpin RNA;
Abstract: Objective To investigate the effects of deleted in liver cancer-1 (DLC-1) and focal adhesion kinase (FAK) on the growth and apoptosis of malignant OVCAR-3 cells.Methods DLC-1 expression plasmid,FAK specific small hairpin RNA (shRNA) expression plasmid,and FAK-shRNA combined DLC-1 expression plasmid were constructed and transfected into OVCAR-3 cells.Real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting were used to detect the expression of DLC-1 and FAK in OVCAR-3 cells,cell proliferation and apoptosis were examined by cell counting kit-8 (CCK-8) kit and flow cytometry,and the expression of phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) was measured by using Western blotting.Results While the DLC-1 expression increased or/and FAK knocked down,the growth of OVCAR-3 cells was obviously inhibited [(0.737 ± 0.094),P ＜0.05],but apoptosis was significantly induced [(15.21 ± 1.05) ％,P ＜ 0.05].In addition,the expression of p-ERK1/2 was notably decreased [(0.151 ±0.010),P ＜0.05].Conclusion DLC1 might be implicated in the growth and apoptosis of malignant tumor cells via its regulation on FAK and downstream ERK signaling.
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