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Effect of pelercan on proliferation of fibroblasts and its relationship with basic fibroblast growth factor
Author(s): 
Pages: 804-807
Year: Issue:  4
Journal: Chinese Journal of Experimental Surgery

Keyword:  FibroblastsPerlecanBasic fibroblast growth factor;
Abstract: Objective To observe the effect of pelercan on proliferation of fibroblasts and its relationship with basic fibroblast growth factor (bFGF).Methods The rat model was established,and the scar connective tissue was collected and subjected to the primary culture of rat fibroblasts.Lentivirus was used to downregulate the expression of pelercan.Fluorescent quantitative polymerase chain reaction (FQ-PCR),Western blotting and methyl thiazol tetrazolium (MTT) assay were performed to detect the expression of perlecan and cell proliferation rate.Results After the lentivirus transfection,the perlecan mRNA expre ssion level in fibroblasts was reduced by (75.1 ± 1.1) % in perlecan-interfering group.In low-serum condition,the cell proliferation rate was decreased by (40.6 ± 3.1)% in perlecan-interfering group.Treatment with 1 μg/L bFGF had no significant effect on the cell proliferation (P > 0.05).Conclusion Infection of rat fibroblasts with lentivirus down-regulated the expression of perlecan and bFGF.Cell proliferation rate and sensitivity of rat fibroblasts to bFGF were both reduced when perlecan was downregulated.
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