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Influence of granulocyte macrophagecolony stimulating factor on the expression of angiopoietin-1 in deep partial thickness burn wound in rats
Author(s): 
Pages: 697-700
Year: Issue:  4
Journal: Chinese Journal of Experimental Surgery

Keyword:  Granulocyte macrophage colony stimulating factorAngiopoietinBurnWound healing;
Abstract: Objective To investigate the influence of granulocyte macrophage colony stimulating factor (GM-CSF) on the expression of angiopoietin-1 (Ang-1) in deep partial thickness burn wound in rats.Methods The models of deep partial thickness burn wounds on the back in Spregue-Dawley (SD) rats were established.The paired wounds on the same rats were randomly divided into GM-CSF treatment (test) and control groups by self-control.Tissue samples were harvested from the wounds on the 1 st,3rd,7th,14th and 21st day respectively after burns.The mRNA expression of Ang-1 was detected by using real-time reverse transcriptase-polymerase chain reaction (FQ-PCR).And the protein expression of Ang-1 was detected by immunohistochemical and western blot,the protein expression of CD31 by using immunohistochemical staining,and the microvascular density was calculated.Results The mRNA expression level of Ang-1 in the burn wounds was decreased slightly on the 1st and 3rd postburn days and that in test group (1.44 ±0.20,0.30 ± 0.17) was lower than that in control group (2.36 ± 0.18,1.34 ± 0.24) (P <0.05).Then the Ang-1 mRNA expression was gradually increased on the 7th and 14th postburn days and decreased slightly on the 21st postburn day,and the expression level in the test group at the above time points (4.52 ±0.21,8.47 ±0.14,7.52 ±0.20) was higher than that in control group (2.71 ±0.12,3.69 ±0.24,3.07 ±0.17) (P <0.05).The protein expression of Ang-1 was declined remarkably on the 1st and 3rd postburn days,which in test group (0.491 ±0.055,0.181 ±0.025) was lower than that in control group (0.735 ±0.080,0.436 ±0.071) (P <0.05).Then the Ang-1 protein expression was gradually increased and reached the peak on the 14th postburn day and the expression in the test group (0.821 ±0.060,1.257 ± 0.225,1.126 ± 0.131) was higher than that in the control group (0.701 ± 0.078,0.825 ± 0.072,0.733 ±0.073) (P <0.05).The microvascular number with positive CD31 expression was increased evidently from the 3rd postburn day in test group,and the counting of MVD was remarkably higher in the test group (9.5±1.8,23.4±2.9,38.4±3.1,24.6±2.5) than in the control group (4.0±1.9,10.5±2.0,22.9 ±2.7,15.1 ±2.6) on the 3rd,7th,14th and 21st days respectively after burns (P <0.05).Conclusion GM-CSF inhibits the mRNA and protein expression of Ang-1 during the early stage of wound healing,which can be beneficial to the angiogenesis.And GM-CSF boosts Ang-1 expression during the middle and late stages of wound healing.This would be helpful to wound vascular maturation and wound healing.
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